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HMC05 attenuates vascular contraction through inhibition of RhoA/Rho-kinase signaling pathway

Authors
Seok, Young MiJin, FanxueShin, Heung-MookSung, Sang HyunSohn, Uy DongCho, Joon YongKim, In Kyeom
Issue Date
Jan-2011
Publisher
ELSEVIER IRELAND LTD
Keywords
HMC05; MYPT1(Thr855); MLC(20); RhoA/Rho-kinase; Vascular contraction; NaF
Citation
JOURNAL OF ETHNOPHARMACOLOGY, v.133, no.2, pp 484 - 489
Pages
6
Journal Title
JOURNAL OF ETHNOPHARMACOLOGY
Volume
133
Number
2
Start Page
484
End Page
489
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21795
DOI
10.1016/j.jep.2010.10.024
ISSN
0378-8741
1872-7573
Abstract
Aim of the study: HMC05, an extract from eight different herbal mixtures, has been developed to treat cardiovascular disease. This extract has a vasorelaxant and anti-atherosclerotic action. We hypothesized that HMC05 attenuates vascular contraction through inhibition of the RhoA/Rho-kinase signaling pathway. Materials and methods: Rat aortic ring preparations were mounted in organ baths and subjected to contraction and relaxation. Phosphorylation of 20 kDa myosin light chains (MLC(20)) and myosin phosphatase targeting subunit 1 (MYPT1) were examined by immunoblot. We also measured the amount of GTP RhoA as a marker for RhoA activation. Results: In endothelium-denuded aortic ring preparations, HMC05 relaxed vascular contraction induced by 6.0 mM NaF, 100 nM phenylephrine, 30 nM thromboxane A(2) agonist U46619 or 1.0 mu M protein kinase C (PKC) activator phorbol-12,13-dibutyrate (PDBu) in a decreasing order. HMC05 relaxed aortic ring preparations precontracted with sodium fluoride (NaF) whether endothelium Was intact or denuded. Pre-incubation with HMC05 for 30 min dose-dependently inhibited the NaF-induced contractile response. In vascular strips, HMC05 decreased the phosphorylation level of both MLC(20) and MYPT1(Thr855) induced by 6.0 mM NaF. Furthermore, HMC05 decreased the amount of GTP RhoA activated by NaF. Conclusions: HMC05 attenuates vascular contraction through inhibition of the RhoA/Rho-kinase signaling pathway. HMC05 may be useful for the treatment and/or prevention of cardiovascular diseases associated with activation of RhoA/Rho-kinase signaling pathway. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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