Chryseobacterium chungangensis sp nov., a bacterium isolated from soil of sweet gourd garden

Abstract

A novel bacterial strain, named MAH-7(T), was isolated from a soil sample of a Korean sweet gourd garden and was characterized using a polyphasic approach. Cells were Gram-staining negative, orange colored, non-motile and rod shaped. The strain was aerobic and catalase, oxidase positive, optimum growth temperature and pH were 28-30 degrees C and 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain MAH-7(T) belongs to the genus Chryseobacterium and is most closely related to Chryseobacterium formosense CC-H3-2(T) (97.96%) and Chryseobacterium zeae JM-1085(T) (97.19%). In DNA-DNA hybridization tests, the DNA relatedness between strain MAH-7(T) and its closest phylogenetic neighbors were below 45.0%. The DNA G+C content was 37.6 mol% and the predominant respiratory quinone was menaquinone-6 (MK-6). Flexirubin-type pigments were found to be present. The major cellular fatty acids were C-15:0 iso,C- C-17:0 iso 3OH, C-17:1 iso omega 9c and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c). The DNA-DNA hybridization results and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain MAH-7(T) represented a novel species within the genus Chryseobacterium, for which the name Chryseobacterium chungangensis is proposed. The type strain is MAH-7(T) (= KACC 19293(T) = CGMCC 1.16232(T)). The NCBI GenBank accession number for the 16S rRNA gene sequence of strain MAH-7(T) is KY964274.