Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells
- Authors
- Song, Ho Sun; Kim, Hee Rae; Ko, Myoung Soo; Jeong, Jae Min; Kim, Yong Ho; Kim, Myung Cheul; Hwang, Yeon Hee; Sohn, Uy Dong; Gimm, Yoon-Myoung; Myung, Sung Ho; Sim, Sang Soo
- Issue Date
- Dec-2010
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- EMF; Phospholipase A(2); Arachidonic acid; Phospholipase C; Phospholipase D
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.14, no.6, pp 427 - 433
- Pages
- 7
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 14
- Number
- 6
- Start Page
- 427
- End Page
- 433
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22030
- DOI
- 10.4196/kjpp.2010.14.6.427
- ISSN
- 1226-4512
2093-3827
- Abstract
- This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A(2) (PLA(2)), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 mu M melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA(2) assay, we failed to observe the change of cPLA(2) and sPLA(2) activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.
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