Effects of Anti-B7.1/B7.2 Antibodies on LPS-Stimulated Macrophages
- Authors
- Won, Tae Joon; Huh, Yoon Joo; Lim, Young Tae; Song, Dong Sup; Hwang, Kwang Woo
- Issue Date
- 31-Oct-2010
- Publisher
- KOREAN SOC APPLIED PHARMACOLOGY
- Keywords
- Anti-B7.1/B7.2 antibodies; RAW264.7; Peritoneal macrophage; Co-stimulatory molecule; Pro-inflammatory cytokine
- Citation
- BIOMOLECULES & THERAPEUTICS, v.18, no.4, pp 463 - 468
- Pages
- 6
- Journal Title
- BIOMOLECULES & THERAPEUTICS
- Volume
- 18
- Number
- 4
- Start Page
- 463
- End Page
- 468
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22133
- DOI
- 10.4062/biomolther.2010.18.4.463
- ISSN
- 1976-9148
2005-4483
- Abstract
- T-cell activation depends on signals received by the T-cell receptor and 0028 co-stimulatory receptor. Since B7.1 and B7.2 molecules expressed on the surface of antigen presenting cells provide co-stimulatory signals through CD28 to T-cells, an inhibitor of CD28-B7.1/B7.2 binding has been proposed as a therapeutic agent for suppression of excessive T-cell activity. Although anti-B7.1/B7.2 antibodies are known to block B7.1 and B7.2 molecules, their effects on intracellular events in antigen presenting cells remain unclear. In this study, anti-B7.1/B7.2 antibodies decreased secretion of nitric oxide and pro-inflammatory cytokines such as TNF-alpha, IL-1 beta, and IL-12 in LPS-activated RAW264.7 macrophage-like cells and peritoneal macrophages. Moreover, anti-B7.1/B7.2 antibodies inhibited I kappa B alpha phosphorylation and down-regulated expression of co-stimulatory molecules including B7.1, B7.2, and PD-L1 in LPS-stimulated peritoneal macrophages. These findings suggest that CTLA4-Ig and anti-B7.1/B7.2 antibodies may be candidates to treat chronic inflammatory diseases and autoimmune responses caused by excessive activation of both T-cells and macrophages.
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