P2X and P2Y Receptors Mediate Contraction Induced by Electrical Field Stimulation in Feline Esophageal Smooth Muscle
- Authors
- Cho, Young Rae; Jang, Hyeon Soon; Kim, Won; Park, Sun Young; Sohn, Uy Dong
- Issue Date
- Oct-2010
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Electrical field stimulation; Smooth muscle; G protein; P2 receptor; ATP; Calcium
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.14, no.5, pp 311 - 316
- Pages
- 6
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 14
- Number
- 5
- Start Page
- 311
- End Page
- 316
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22157
- DOI
- 10.4196/kjpp.2010.14.5.311
- ISSN
- 1226-4512
2093-3827
- Abstract
- It is well-known that electrical field stimulation (EFS)-induced contraction is mediated by a cholinergic mechanism and other neurotransmitters NO, ATP, calcitonin gene-related peptide (CGRP), and substance P are released by EFS To investigate the purinergic mechanism involved in the EFS-induced contraction, purinegic receptors antagonists were used Suramine, a non-selective P2 receptor antagonist, reduced the contraction induced by EFS NF023 (10(-7) similar to 10(-4) M), a selective P2X antagonist, inhibited the contraction evoked by EFS Reactive blue (10(-6) similar to 10(-4) M), selective P2Y antagonist, also blocked the contraction in a dose-dependent manner In addition, P2X agonist alpha,beta-methylene 5`-adenosine triphosphate (alpha beta MeATP, 10(-7) similar to 10(-5) M) potentiated EFS-induced contraction in a dose-dependent manner P2Y agonist adenosine 5`[beta -thio]diphosphate trilithium salt (ADP beta S, 10(-7) similar to 10(-5) M) also potentiated EFS-induced contractions in a dose-dependent manner Ecto-ATPase activator apyrase (5 and 10 U/ml) reduced EFS-induced contractions Inversely, 6-N,N-diethyl-D-beta,gamma- dibromomethylene 5`-triphosphate triammonium (ARL 67156, 10(-4) M) increased EFS-induced contraction These data suggest that endogenous ATP plays a role in EFS-induced contractions which are mediated through both P2X-receptors and P2Y-receptors stimulation in cat esophageal smooth muscle
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