A promoter polymorphism -2122C > T of CHI3L1 is associated with serum low density lipoprotein cholesterol level in Korean subjects

Abstract

Objectives: This study assessed the effects of 10 tagging single nucleotide polymorphisms (SNPs) of the CHI3L1 gene on serum LDL cholesterol levels in 290 Korean subjects. Design and methods: Genotyping analyses of SNPs were conducted by TagMan (R) method. The effects of the promoter SNP on mRNA expression and nuclear factor binding were measured by real-time PCR and electrophoretic mobility shift assay, respectively. Results: Among 10 tagging SNPs, - 2122C>T SNP (rs946261) in the promoter region was significantly associated with serum LDL cholesterol level (P=0.005). The T allele of - 2122C>T was associated with significantly increased mRNA expressions in peripheral blood cells of the subjects, and also increased a nuclear factor binding measured by an electrophoretic mobility shift assay. Conclusions: - 2122C>T of CHI3L1, a promoter SNP which affects the mRNA expression and nuclear factor binding, is significantly associated with serum LDL cholesterol levels in Korean subjects. (C) 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.