Functional Relationships between the AcrA Hairpin Tip Region and the TolC Aperture Tip Region for the Formation of the Bacterial Tripartite Efflux Pump AcrAB-TolCopen access
- Authors
- Kim, Hong-Man; X, Yongbin; Lee, Minho; Piao, Shunfu; Sim, Se-Hoon; Ha, Nam-Chul; Lee, Kangseok
- Issue Date
- Sep-2010
- Publisher
- AMER SOC MICROBIOLOGY
- Citation
- JOURNAL OF BACTERIOLOGY, v.192, no.17, pp 4498 - 4503
- Pages
- 6
- Journal Title
- JOURNAL OF BACTERIOLOGY
- Volume
- 192
- Number
- 17
- Start Page
- 4498
- End Page
- 4503
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22231
- DOI
- 10.1128/JB.00334-10
- ISSN
- 0021-9193
1098-5530
- Abstract
- Tripartite efflux pumps found in Gram-negative bacteria are involved in antibiotic resistance and toxicprotein secretion. In this study, we show, using site-directed mutational analyses, that the conserved residues located in the tip region of the alpha-hairpin of the membrane fusion protein (MFP) AcrA play an essential role in the action of the tripartite efflux pump AcrAB-TolC. In addition, we provide in vivo functional data showing that both the length and the amino acid sequence of the alpha-hairpin of AcrA can be flexible for the formation of a functional AcrAB-TolC pump. Genetic-complementation experiments further indicated functional interrelationships between the AcrA hairpin tip region and the TolC aperture tip region. Our findings may offer a molecular basis for understanding the multidrug resistance of pathogenic bacteria.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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