Hemolysin Gene Expression in the hns Knockout Mutant of Klebsiella pneumoniae UN Strain
- Authors
- Han, Jang Suk; Jang, In-Young; Ryu, Hyung-Seok; Kim, Woo-Yeon
- Issue Date
- Aug-2010
- Publisher
- KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
- Keywords
- hemolysin; H-NS; Klebsiella pneumoniae UN
- Citation
- JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY, v.53, no.4, pp 401 - 406
- Pages
- 6
- Journal Title
- JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY
- Volume
- 53
- Number
- 4
- Start Page
- 401
- End Page
- 406
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22313
- DOI
- 10.3839/jksabc.2010.062
- ISSN
- 1738-2203
2234-344X
- Abstract
- Hemolysin proteins are exotoxins produced by bacteria that can cause lysis of red blood cells in vitro. Hemolysin E is a pore-forming hemolysin, one of three types of hemolysin. Generally, hemolysin E is not expressed under standard growth condition's such as growth in LB medium at 37 degrees C. However, in E. coli, when the hns gene, which encodes H-NS, a global regulatory DNA binding protein, is knocked out, hemolysin E gene is expressed. Because hns genes in E. coli and K. pneumoniae are similar, K pneumoniae UN strain hns gene was knocked out in this research. The hns knockout mutant of K. pneumoniae UN is found to produce a 19 kDa hemolysin protein and shows hemolytic activity.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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