Simultaneous Separation and Determination of Isosorbide Dinitrate and Isosorbide 5-Mononitrate in Human Plasma by LC-MS-MS
- Authors
- Kim, Hohyun; Lee, Jae-Yong; Song, Ki-Seob; Seo, Young-Hwan; Park, So-Young; Kim, Kyeong-Ho; Je, Hyun Dong; Han, Sang Beom; Jeong, Ji Hoon
- Issue Date
- Apr-2010
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Column liquid chromatography; Tandem mass spectrometry; Pharmacokinetic study; Isosorbide dinitrate and isosorbide 5-mononitrate
- Citation
- CHROMATOGRAPHIA, v.71, no.7-8, pp 595 - 602
- Pages
- 8
- Journal Title
- CHROMATOGRAPHIA
- Volume
- 71
- Number
- 7-8
- Start Page
- 595
- End Page
- 602
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22551
- DOI
- 10.1365/s10337-010-1480-6
- ISSN
- 0009-5893
- Abstract
- A sensitive, selective, and simple liquid chromatography-tandem mass spectrometry method has been developed for the simultaneous separation and determination of isosorbide dinitrate and its active metabolite, isosorbide 5-mononitrate, in human plasma. Topiramate was used as the internal standard. Sample preparation consisted of a simple one-step liquid-liquid extraction with ethylacetate without pH adjustment. The method was fully validated with respect to linearity, sensitivity, specificity, recovery, accuracy, and precision. Isosorbide dinitrate and isosorbide 5-mononitrate were stable in standard solution and in plasma samples under storage and processing conditions. The assay was successfully applied to the pharmacokinetic study of isosorbide dinitrate and isosorbide 5-mononitrate in human plasma.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
- College of Medicine > College of Medicine > 1. Journal Articles
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