MLCK and PKC Involvements via Gi and Rho A Protein in Contraction by the Electrical Field Stimulation in Feline Esophageal Smooth Muscle
- Authors
- Park, Sun Young; Shim, Je Ho; Kim, Mina; Sun, Yih Hsiu; Kwak, Hyun Soo; Yan, Xiangmai; Choi, Byung-Chul; Im, Chaeuk; Sim, Sang Soo; Jeong, Ji Hoon; Kim, In Kyeom; Min, Young Sil; Sohn, Uy Dong
- Issue Date
- Feb-2010
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Electrical field stimulation; Smooth muscle; Ca2+; K+; G protein; On contraction; Esophagus
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.14, no.1, pp 29 - 35
- Pages
- 7
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 14
- Number
- 1
- Start Page
- 29
- End Page
- 35
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22623
- DOI
- 10.4196/kjpp.2010.14.1.29
- ISSN
- 1226-4512
2093-3827
- Abstract
- We have shown that myosin light chain kinase (MLCK),vas required for the off-contraction in response to the electrical field stimulation (EFS) of feline esophageal smooth muscle. In this study, we investigated whether protein kinase C (PKC) may require the on-contraction in response to EFS using feline esophageal smooth muscle. The contractions were recorded using an isometric force transducer. On-contraction occurred in the presence of N-G-nitro-L-arginine methyl ester (L-NAME), suggesting that nitric oxide acts as an inhibitory mediator in smooth muscle. The excitatory composition of both contractions was cholinergic dependent which was blocked by tetrodotoxin or atropine. The on-contraction was abolished in Ca2+-free buffer but reappeared in normal Ca2+-containing buffer indicating that the contraction was Ca2+ dependent. 4-aminopyridine (4-AP), voltage-dependent K+ channel blocker, significantly enhanced on-contraction. Aluminum fluoride (a G-protein activator) increased on-contraction. Pertussis toxin (a G(i) inactivator) and C3 exoenzyme (a rhoA inactivator) significantly decreased on-contraction suggesting that Gi or rhoA protein may be related with Ca2+ and K+ channel. ML-9, a MLCK inhibitor, significantly inhibited on-contraction, and chelerythrine (PKC inhibitor) affected on the contraction. These results suggest that endogenons cholinergic contractions activated directly by low-frequency EFS may be mediated by Ca2+, and G proteins, such as Gi and rhoA, which resulted in the activation of MLCK, and PKC to produce the contraction in feline distal esophageal smooth muscle.
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Collections - College of Medicine > College of Medicine > 1. Journal Articles
- College of Pharmacy > School of Pharmacy > 1. Journal Articles
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