Modulation of human beta-defensin-2 expression by 17 beta-estradiol and progesterone in vaginal epithelial cells
- Authors
- Han, June Hyun; Kim, Min Su; Lee, Moo Yeol; Kim, Tae Hyoung; Lee, Mi-Kyung; Kim, Hye Ryoun; Myung, Soon Chul
- Issue Date
- Feb-2010
- Publisher
- ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
- Keywords
- Vagina; Epithelial cells; Defensins; Vaginitis; Urinary tract infection
- Citation
- CYTOKINE, v.49, no.2, pp 209 - 214
- Pages
- 6
- Journal Title
- CYTOKINE
- Volume
- 49
- Number
- 2
- Start Page
- 209
- End Page
- 214
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22656
- DOI
- 10.1016/j.cyto.2009.09.005
- ISSN
- 1043-4666
1096-0023
- Abstract
- We investigated the expression of HBD-1 and -2 in vaginal epithelial cells treated with lipopolysaccharide (LPS) and the effects on HBD-2 expressions by 17 beta-estradiol and progesterone. Primary vaginal epithelial cells were isolated from a segment of normal anterior vaginal wall obtained during vaginoplasty and were cultured in keratinocyte growth medium and were allowed to undergo their 3rd passage. Expression of HBD-1 and -2 by different stimuli using LPS 0.5 mu g/ml, 17 beta-estradiol 2 nM and progesterone 1 mu M was measured by RT-PCR, ELISA and real-time RT-PCR, respectively. HBD-1 was produced constitutively in vaginal epithelial cells and the production of HBD-1 was not influenced by LPS, 17 beta-estradiol and progesterone, but the production of HBD-2 was increased inducibly by LPS. 17 beta-Estradiol and progesterone did not change the production of HBD-2 in normal state, but 17 beta-estradiol increased the production of HBD-2 and progesterone suppressed the production of HBD-2 under the circumstances with infection. The HBD-2 plays an important role at innate host defense on genitourinary tract. The lacks of estrogen during menopause or uses of a progesterone-based oral contraceptive in sexually active women may influence production of HBD-2 in vaginal epithelium and may increase susceptibility to bacterial vaginitis or recurrent UTI. (C) 2009 Elsevier Ltd. All rights reserved.
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