Promigratory and procontractile growth factor environments differentially regulate cell morphogenesisopen access
- Authors
- Rhee, Sangmyung; Ho, Chin-Han; Grinnell, Frederick
- Issue Date
- Jan-2010
- Publisher
- ELSEVIER INC
- Keywords
- Platelet-derived growth factor; Lysophosphatidic acid; 3D collagen matrix; Cell motility; Cell contraction
- Citation
- EXPERIMENTAL CELL RESEARCH, v.316, no.2, pp 232 - 244
- Pages
- 13
- Journal Title
- EXPERIMENTAL CELL RESEARCH
- Volume
- 316
- Number
- 2
- Start Page
- 232
- End Page
- 244
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22673
- DOI
- 10.1016/j.yexcr.2009.09.021
- ISSN
- 0014-4827
1090-2422
- Abstract
- Three-dimensional (3D) cell-matrix cultures provide a useful model to analyze and dissect the structural, functional, and mechanical aspects of cell-matrix interactions and motile behavior important for cell and tissue morphogenesis. In the current studies we tested the effects of serum and physiological growth factors on the morphogenetic behavior of human fibroblasts cultured on the surfaces of 3D Collagen matrices. Fibroblasts in medium containing serum contracted into clusters, whereas cells in medium containing platelet-derived growth factor (PDGF) were observed to migrate as individuals. The clustering activity of serum appeared to depend on lysophosphatidic acid, required cell contraction based on inhibition by blocking Rho kinase or myosin 11, and was reversed upon switching to PDGF. Oncogenic Ras transformed human fibroblasts did not exhibit serum-stimulated cell clustering. Our findings emphasize the importance of cell-specific promigratory and procontractile growth factor environments in the differential regulation of cell motile function and cell morphogenesis. (C) 2009 Elsevier Inc. All rights reserved.
- Files in This Item
-
- Appears in
Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.