Anti-inflammatory and anti-oxidant effects of Sophora flavescens root extraction in lipopolysaccharide-activated raw 264.7 cells
- Authors
- Dong, H.L.; Dong, S.S.; Dai, Y.C.; Beom, J.K.; Yun, Y.L.; Young, H.K.
- Issue Date
- 1-Jun-2010
- Publisher
- Korean Society for Medical Mycology
- Keywords
- COX-2; iNOS; Sophora flavescens
- Citation
- Korean Journal of Medical Mycology, v.15, no.2, pp 39 - 50
- Pages
- 12
- Journal Title
- Korean Journal of Medical Mycology
- Volume
- 15
- Number
- 2
- Start Page
- 39
- End Page
- 50
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22737
- ISSN
- 1226-4709
2465-8278
- Abstract
- Background: The macrophages activated by lipopolisaccharide produce numerous molecules and proteins, such as tumor necrotic factor-α (TNF-α), interleukin-6 (IL-6), IL-β, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and free radicals, associated with inflammation. The response was occurred by intracellular cascaded, NF-κB pathway. So, the regulation of this pathway is very important in control of inflammation. Objective: In this study, the anti-inflammatory and anti-oxidant effects of Sophora flavescens that is used empirically in oriental medicine and folk remedy were evaluated and the mechanism of the effects was studied. Methods: By using the root extracts of Sophora flavescens, we performed experiment in LPS and interferon-γ (IFN-γ)-activated Raw 264.7 cells. We measured the production of NO, PGE 2 and expression of iNOS and COX-2 in activated Raw 264.7 cells with Sophora flavescens root extract. Also, we tested anti-oxidant effect of Sophora flavescens root extracts by ELISA kit in activated Raw 264.7 cells, and the free radical scavenging effect of material itself by DPPH assay. Results: The Sophora flavescens root extracts decreased the production of NO (p<0.001) and PGE 2 (p<0.01) in Raw 264.7 cells activated by LPS and IFN-γ. The expression of proteins, iNOS and COX-2, suppressed along with the elevated concentration of Sophora flavescens root extracts. The result of DPPH assay was that the test material itself had scavenging effect for free radical (p<0.001). And the antioxidant activity in activated Raw 264.7 cells was increased with the level of the Sophora flavescens root extracts (p<0.05). Conclusion: The Sophora flavescens root extracts suppressed the production of NO and PGE 2 through the decreased expression of iNOS and COX-2. And the Sophora flavescens root extracts had the scavenging effect about free radicals itself and increased the antioxidant activity in activated macrophages.
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