2 '-Hydroxylation of Genistein Enhanced Antioxidant and Antiproliferative Activities in MCF-7 Human Breast Cancer Cells
- Authors
- Choi, Jung Nam; Kim, Dockyu; Choi, Hyung Kyoon; Yoo, Kyung Mi; Kim, Jiyoung; Lee, Choong Hwan
- Issue Date
- Nov-2009
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- Genistein; 2 '-hydroxygenistein; bioconversion; antioxidant activity; antiproliferation activity; MCF-7 cells
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.19, no.11, pp 1348 - 1354
- Pages
- 7
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 19
- Number
- 11
- Start Page
- 1348
- End Page
- 1354
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22926
- DOI
- 10.4014/jmb.0903.0114
- ISSN
- 1017-7825
1738-8872
- Abstract
- Bioconversion of the isoflavonoid genistein to 2'-hydroxygenistein (2'-HG) was performed using isoflavone 2'-hydroxylase (CYP81E1) heterologously expressed in yeast. A monohydroxylated product was analyzed by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and NMR spectrometry and was identified as 2'-HG. An initial bioconversion rate of 60% was increased up to 14% under optimized conditions. After recovery, the biological activity of 2'-HG was evaluated. Bioconverted 2'-HG showed higher antioxidant activity against 1,1-diphenyl-2-picryl hydrazine (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals than did genistein. Furthermore, 2'-HG exhibited greater antiproliferative effects in MCF-7 human breast cancer cells than did genistein. These results suggest that 2'-hydroxylation of genistein enhanced its antioxidant activity and cell cytotoxicity in MCF-7 human breast cancer cells.
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