Diarylheptanoid hirsutenone prevents tumor necrosis factor-alpha-stimulated production of inflammatory mediators in human keratinocytes through NF-kappa B inhibition
- Authors
- Lee, Chung Soo; Ko, Hyun Hee; Seo, Seong Jun; Choi, Young Wook; Lee, Min Won; Myung, Soon Chul; Bang, Hyoweon
- Issue Date
- Aug-2009
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Keratinocytes; Tumor necrosis factor-alpha; Hirsutenone; NF-kappa B; Cytokine and chemokine production; Inhibitory effect
- Citation
- INTERNATIONAL IMMUNOPHARMACOLOGY, v.9, no.9, pp 1097 - 1104
- Pages
- 8
- Journal Title
- INTERNATIONAL IMMUNOPHARMACOLOGY
- Volume
- 9
- Number
- 9
- Start Page
- 1097
- End Page
- 1104
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23067
- DOI
- 10.1016/j.intimp.2009.05.006
- ISSN
- 1567-5769
1878-1705
- Abstract
- Keratinocytes may play an important role in the pathogenesis of skin disease in atopic dermatitis. Diarylheptanoids such as oregonin and hirstanonol are demonstrated to have anti-inflammatory and antioxidant effects. The present study was to investigate the effect of hirsutenone, one of the diarylheptanoids, against tumor necrosis factor (TNF)-alpha-stimulated responses in human keratinocytes. Hirsutenone attenuated the TNIF-alpha-induced production of cytokine IL-8, prostaglandin E-2 and chemokine CCL27, and the formation of reactive oxygen/nitrogen species in keratinocytes. Immunosuppressants (dexamethasone and cyclosporin A) inhibited the TNF-alpha-elicited formation of IL-8, prostaglandin E-2 and CCL27, but did not affect formation of reactive species. Bay 11-7085 (an inhibitor of NF-kappa B activation) and anti-oxidant N-acetylcysteine attenuated the TNF-alpha-induced formation of inflammatory mediators and reactive species. Hirsutenone, dexamethasone, cyclosporin A and Bay 11-7085 inhibited the TNF-alpha-induced phosphorylation of inhibitory kappa B and the activation of nuclear factor (NF)-kappa B. The results show that hirsutenone seems to reduce the TNF-alpha-stimulated production of inflammatory mediators in keratinocytes by suppressing the activation of NF-kappa B that may be mediated by reactive oxygen species. The findings suggest that hirsutenone may exert an inhibitory effect against the pro-inflammatory mediator-induced skin disease. (C) 2009 Elsevier B.V. All rights reserved.
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Collections - College of Medicine > College of Medicine > 1. Journal Articles
- College of Pharmacy > School of Pharmacy > 1. Journal Articles
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