Immunoblotting reactivity of vitellogenin antibodies against native vitellogenins and a vitellogenin protein fragment produced in e. coliImmunoblotting Reactivity of Vitellogenin Antibodies against Native Vitellogenins and a Vitellogenin Protein Fragment Produced in E. coli
- Authors
- Ryu, H.-S.; Jang, I.-Y.; Kim, W.-Y.
- Issue Date
- 2009
- Publisher
- 한국응용생명화학회
- Keywords
- Antibody; Flatfish vitellogenin; Reactivity
- Citation
- Journal of Applied Biological Chemistry, v.52, no.4, pp 170 - 173
- Pages
- 4
- Journal Title
- Journal of Applied Biological Chemistry
- Volume
- 52
- Number
- 4
- Start Page
- 170
- End Page
- 173
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23455
- DOI
- 10.3839/jabc.2009.029
- ISSN
- 1976-0442
2234-7941
- Abstract
- Vitellogenin (Vtg) is found in the serum of both female and male fish that have been exposed to environmental endocrine disrupters or estrogen hormones, and is used as a biomarker for such contamination. In our current study antibodies raised against the purified flatfish Vtg were tested for their reactivity on immunoblots to flatfish and carp Vtg, and also to a Vtg protein fragment produced in E. coli. Polyclonal antibodies raised against purified flatfish Vtg reacted well with Vtg in the serum of flatfish and carp induced with 17β-estradiol, but not with the Vtg protein fragment produced in E. coli.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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