Optimal culture condition for antifungal susceptibility tests of Malassezia globosa

Abstract

Background: Although numerous culture conditions for Malassezia species were suggested, there were not so many objective evaluation articles in the literature. Objectives: We examined the various culture conditions for Malassezia globosa. Methods: Malassezia globosa culture conditions were assessed by Dixon's agar, modified Leeming-Notman medium in diverse oil content and temperature conditions. Results: Maximum growth rate of Malassezia globosa was achieved at 3% olive oil. The optimal temperatures for the maximal growth of M. globosa were observed at 32-34°C. Conclusion: In this study, we established the optimal culture condition for M. globosa, and confirmed its excellent utility for the antifungal susceptibility tests for M. globosa and M. restricta. Our results can help the investigators plan to do the prospective researches involving Malassezia species, such as the susceptibility test for newly developed antifungal agents.