Pseudoxanthomonas sacheonensis sp nov., isolated from BTEX-contaminated soil in Korea, transfer of Stenotrophomonas dokdonensis Yoon et al. 2006 to the genus Pseudoxanthomonas as Pseudoxanthomonas dokdonensis comb. nov and emended description of the genus Pseudoxanthomonas

Abstract

A Gram-negative, strictly aerobic, rod-shaped bacterium, designated strain BD-c54(T), was isolated from BTEX (benzene, toluene, ethylbenzene and xylenes)-contaminated soil in Sacheon, Korea. Growth of strain BD-c54(T) was observed at 15-35 degrees C (optimum 25-30 degrees C and pH 6.0-9.5 (optimum pH 7.0-8.0). The predominant fatty acids were iso-C-15:0, iso-C-17:1 omega 9c, iso-C-11:0 3-OH, iso-C-16:0, iso-C-11:0 and iso-C-17:0. The strain contained large amounts of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol and a small amount of an unknown amino-group-containing polar lipid as polar lipids. The major quinone was ubiquinone-8 (Q-8) and the G + C content of the genomic DNA was 67.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain BD-c54(T) formed a tight phylogenetic lineage with Pseudoxanthomonas yeongjuensis GR12-1(T) within the genus Pseudoxanthomonas and was most closely related to P. yeongjuensis GR12-1(T) and [Stenotrophomonas] dokdonensis DS-16(T), with 98.3 and 96.6% 16S rRNA gene sequence similarity, respectively. The DNA-DNA relatedness between strain BD-c54(T) and P. yeongjuensis GR12-1(T) was 24.5%. On the basis of chemotaxonomic data and molecular properties, strain BD-c54(T) represents a novel species within the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas sacheonensis sp. nov. is proposed. The type strain is BD-c54(T) (=KCTC 22080(T) =DSM 19373(T)). In addition, the transfer of Stenotrophomonas dokdonensis to Pseudoxanthomonas as Pseudoxanthomonas dokdonensis comb. nov. and an emended description of the genus Pseudoxanthomonas are proposed.