Flatfish vitellogenin detection using optical waveguide lightmode spectroscopy-based immunosensor
- Authors
- Kim; Namsow; Ryu, Hyung-Seok; Kim, Woo-Yeon
- Issue Date
- Sep-2007
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- optical waveguide lightmode spectroscopy-based immunosensor; sensitive detection; flatfish vitellogenin
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.9, pp 1445 - 1451
- Pages
- 7
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 17
- Number
- 9
- Start Page
- 1445
- End Page
- 1451
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/23992
- ISSN
- 1017-7825
1738-8872
- Abstract
- A sensitive optical waveguide lightmode spectroscopy-based irnmunosensor was developed to detect vitellogenin in seawater flatfish (Paralichthys olivaceus). For this purpose, anion-exchange column chromatography with DE-52 resin was used to purify flatfish vitellogenin from flatfish serum containing vitellogenin that had been induced using an intraperitorical 17 beta-estradiol injection. The anti-flatfish vitellogenin antibody used as the biological component of the above iinmunosensor was prepared using the purified flatfish vitellogenin. The change in the incoupling angle according to the complexation between the flatfish vitellogenin and its antibody, immobilized over an optical grating coupler sensor chip, was measured to calculate the sensor response. The immunosensor was quite specific to flatfish vitellogenin binding, based on no sensor response in the case of bovine serum albumin immobilization. When plotted using double-logarithmic scales, the sensor responses increased linearly in flatfish vitellogenin concentrations of 0.00675-67.5 nM, with a detection limit of 0.0675 nM. The reusability during seven repetitive measurements was reasonably fair for the preliminary screening of flatfish vitellogenin.
- Files in This Item
-
- Appears in
Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.