Neural differentiation of novel multipotent progenitor cells from cryopreserved human umbilical cord blood
- Authors
- Lee, Myoung Woo; Moon, Young Joon; Yang, Mal Sook; Kim, Sun Kyung; Jang, In Keun; Eom, Young-woo; Park, Joon Seong; Kim, Hugh C.; Song, Kye Yong; Park, Soon Cheol; Lim, Hwan Sub; Kim, Young Jin
- Issue Date
- Jun-2007
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- Differentiation; Isolation; Multipotent progenitor cell; Neuron; Umbilical cord blood
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.358, no.2, pp 637 - 643
- Pages
- 7
- Journal Title
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- Volume
- 358
- Number
- 2
- Start Page
- 637
- End Page
- 643
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24047
- DOI
- 10.1016/j.bbrc.2007.04.181
- ISSN
- 0006-291X
1090-2104
- Abstract
- Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells, with practical and ethical advantages. To date, the presence of other stem cells in UCB remains to be established. We investigated whether other stem cells are present in cryopreserved UCB. Seeded mononuclear cells formed adherent colonized cells in optimized culture conditions. Over a 4- to 6-week culture period, colonized cells gradually developed into adherent mono-layer cells, which exhibited homogeneous fibroblast-like morphology and immunophenotypes, and were highly proliferative. Isolated cells were designated 'multipotent progenitor cells (MPCs)'. Under appropriate conditions for 2 weeks, MPCs differentiated into neural tissue-specific cell types, including neuron, astrocyte, and oligodendrocyte. Differentiated cells presented their respective markers, specifically, NF-L and NSE for neurons, GFAP for astrocytes, and myelin/oligodendrocyte for oligodendrocytes. In this study, we successfully isolated MPCs from cryopreserved UCB, which differentiated into the neural tissue-specific cell types. These findings suggest that cryopreserved human UCB is a useful alternative source of neural progenitor cells, such as NIPCs, for experimental and therapeutic applications. (C) 2007 Elsevier Inc. All rights reserved.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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