Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine
DC Field | Value | Language |
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dc.contributor.author | Yang, Zheng-You | - |
dc.contributor.author | Shim, Won-Bo | - |
dc.contributor.author | Kim, Min-Gon | - |
dc.contributor.author | Lee, Kyu-Ho | - |
dc.contributor.author | Kim, Keun-Sung | - |
dc.contributor.author | Kim, Kwang-Yup | - |
dc.contributor.author | Kim, Cheol-Ho | - |
dc.contributor.author | Ha, Sang-Do | - |
dc.contributor.author | Chung, Duck-Hwa | - |
dc.date.available | 2019-05-30T06:34:10Z | - |
dc.date.issued | 2007-04 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.issn | 1738-8872 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24107 | - |
dc.description.abstract | A monoclonal antibody (mab) against the antimicrobial sulfamethazine was prepared and characterized by an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA). Sulfamethazine in the range of 0.2 and 45 ng/ml could be determined with the mab by IC-ELISA. cDNAs encoding a variable heavy chain and variable light chain of the mab were cloned to produce recombinant antibodies using phage display technology. Following phage rescue and three rounds of panning, a single-chain variable fragment (scFv) antibody with high sulfamethazine-binding affinity was obtained. ELISA analysis revealed that scFv antibody and parent mab showed similar, but not identical, characteristics. The IC,, value by IC-ELISA with scFv antibody was 4.8 ng/ml, compared with 1.6 ng/ml with the parent mab. Performances of the assays in the presence of milk matrix were compared; the mab-based assay was less affected than the scFv-based assay. Sixty milk samples were analyzed by mab-based IC-ELISA, and four samples were sulfamethazine positive; these results were favorably correlated with those obtained by HPLC. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.title | Production and characterization of monoclonal and recombinant antibodies against antimicrobial sulfamethazine | - |
dc.type | Article | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.4, pp 571 - 578 | - |
dc.identifier.kciid | ART001051256 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000246096700003 | - |
dc.identifier.scopusid | 2-s2.0-34248346368 | - |
dc.citation.endPage | 578 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 571 | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 17 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | ELISA | - |
dc.subject.keywordAuthor | sulfamethazine | - |
dc.subject.keywordAuthor | antimicrobial | - |
dc.subject.keywordAuthor | monoclonal antibody | - |
dc.subject.keywordAuthor | scFv antibody | - |
dc.subject.keywordPlus | SINGLE-CHAIN ANTIBODY | - |
dc.subject.keywordPlus | ENZYME-IMMUNOASSAY | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | CHROMATOGRAPHY | - |
dc.subject.keywordPlus | SULFONAMIDES | - |
dc.subject.keywordPlus | FRAGMENTS | - |
dc.subject.keywordPlus | PHAGE | - |
dc.subject.keywordPlus | MILK | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
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