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Interaction proteome analysis of Xanthomonas Hrp proteins
- Authors
- Jang, Mi; Park, Byoung Chul; Lee, Do Hee; Bae, Kwang-Hee; Cho, Sayeon; Park, Hyun Seok; Lee, Baek Rak; Park, Sung Goo
- Issue Date
- Feb-2007
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- hrp gene; interaction protein; 1-DE; MALDI-TOF; pathogenesis
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.2, pp 359 - 363
- Pages
- 5
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 17
- Number
- 2
- Start Page
- 359
- End Page
- 363
- ISSN
- 1017-7825
1738-8872
- Abstract
- Because of the importance of the type III protein-secretion system in bacteria-plant interaction, its function in bacterial pathogenesis of plants has been intensively studied. To identify bacterial proteins interacting with Xanthomonas hrp gene products that are involved in pathogenicity, we performed the glutathione-bead binding analysis of Xanthomonas lysates containing GST-tagged Hrp proteins. Analysis of glutathione-bead bound proteins by I-DE and MALDI-TOF has demonstrated that Avr proteins, RecA, and several components of the type III secretion system interact with HrpB protein. This proteomic approach could provide a powerful tool in finding interaction partners of Hrp proteins whose roles in host-pathogen interaction need further studies.
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