Indole-3-carbinol enhances ultraviolet B-induced apoptosis.by sensitizing human melanoma cells
- Authors
- Kim, D-S.; Jeong, Y-M.; Moon, S-I.; Kim, S-Y.; Kwon, S-B.; Park, E-S.; Youn, S-W; Park, K-C
- Issue Date
- Nov-2006
- Publisher
- BIRKHAUSER VERLAG AG
- Keywords
- ultraviolet B; indole-3-carbinol; melanoma; apoptosis; cancer
- Citation
- CELLULAR AND MOLECULAR LIFE SCIENCES, v.63, no.22, pp 2661 - 2668
- Pages
- 8
- Journal Title
- CELLULAR AND MOLECULAR LIFE SCIENCES
- Volume
- 63
- Number
- 22
- Start Page
- 2661
- End Page
- 2668
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24255
- DOI
- 10.1007/s00018-006-6306-1
- ISSN
- 1420-682X
1420-9071
- Abstract
- Indole-3-carbinol (13C) has been found to act against several types of cancer, while ultraviolet B (UVB) is known to induce the apoptosis of human melanoma cells. Here, we investigated whether 13C can sensitize G361 human melanoma cells to UVB-induced apoptosis. We examined the effects of combined 13C and UVB (13C/UVB) at various dosages. 13C (200 mu M)/UVB (50 mJ/cm(2)) synergistically reduced melanoma cell viability, whereas 13C (200 mu M) or UVB (50 MJ/cm(2)), separately, had little effect on cell viability DNA fragmentation assays indicated that 13C/UVB induced apoptosis. Further results show that 13C/UVB activates caspase-8, -3, and Bid and causes the cleavage of poly(ADP-ribose) polymerase. Moreover, 13C decreased the expression of the anti-apoptotic protein, Bcl-2, whereas UVB increased the translocation of Bax to mitochondria. Thus, an increased Bax/Bcl-2 ratio by 13C/UVB may result in melanoma apoptosis. In conclusion, our study demonstrated that 13C sensitizes human melanoma cells by down-regulating Bcl-2.
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