H32, a non-quinone sulfone analog of vitamin K3, inhibits human hepatoma cell growth by inhibiting Cdc25 and activating ERK
- Authors
- Kar, Siddhartha; Wang, Meifang; Ham, Seung Wook; Carr, Brian I.
- Issue Date
- Oct-2006
- Publisher
- LANDES BIOSCIENCE
- Keywords
- hepatoma cells; small molecule inhibitor; Cdc25 inhibition; ERK phosphorylation
- Citation
- CANCER BIOLOGY & THERAPY, v.5, no.10, pp 1340 - 1347
- Pages
- 8
- Journal Title
- CANCER BIOLOGY & THERAPY
- Volume
- 5
- Number
- 10
- Start Page
- 1340
- End Page
- 1347
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24274
- DOI
- 10.4161/cbt.5.10.3223
- ISSN
- 1538-4047
1555-8576
- Abstract
- We previously synthesized a K-vitamin derivative, Cpd 5, which was a potent growth inhibitor of human tumor cells, including Hep3B hepatoma cells. However, being a quinone compound, Cpd 5 has the potential for generating toxic reactive oxygen species (ROS). We therefore synthesized a nonquinone sulfone derivative, H32, which has a sufone group substituting the quinone. The IC50 of H32 for Hep3B cells was found to be 2.5 mu M, which was 2.5 and 3.2 times more potent than Cpd 5 and vitamin K3 respectively. It induced apoptosis in Hep3B cells but did not generate ROS when compared to Cpd 5. Interestingly, under similar culture conditions, normal rat hepatocytes were 14-fold more and 7-fold more resistant to the growth inhibitory effects of H32 than Hep3B and PLC/ PRF5 cells respectively. H32 preferentially inhibited the activities of the cell cycle controlling Cdc25A phosphatase likely by binding to its catalytic cysteine. As a consequence, it induced inhibitory tyrosine phosphorylation of the Cdc25 substrate kinases Cdk2 and Cdk4 in Hep3B cells and the cells undergo an arrest in the G(1) phase of the cell cycle. H32 also induced persistent phosphorylation of the MAPK protein ERK1/2, but marginal JNK1/2 and p38 phosphorylation. The ERK inhibitor U0126, added at least 30 min prior to H32, antagonized the growth inhibition induced by H32. However, the JNK and p38 inhibitors, JNKI-II and SB203580, were not able to antagonize H32 induced growth inhibition. Thus, H32 differentially inhibited growth of normal and liver tumor cells by preferentially inhibiting the actions of Cdc25 phosphatases and inducing persistent ERK phosphorylation.
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