Differential involvement of intracellular Ca2+ in 1-methyl-4-phenylpyridinium- or 6-hydroxydopamine-induced cell viability loss in PC12 cells
- Authors
- Lee, Dong Hee; Han, Young Su; Han, Eun Sook; Bang, Hyoweon; Lee, Chung Soo
- Issue Date
- Jul-2006
- Publisher
- SPRINGER/PLENUM PUBLISHERS
- Keywords
- 1-methyl-4-phenylpyridinium; 6-hydroxydopamine; mitochondrial membrane permeability; intracellular Ca2+ homeostasis; PC12 cells
- Citation
- NEUROCHEMICAL RESEARCH, v.31, no.7, pp 851 - 860
- Pages
- 10
- Journal Title
- NEUROCHEMICAL RESEARCH
- Volume
- 31
- Number
- 7
- Start Page
- 851
- End Page
- 860
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24310
- DOI
- 10.1007/s11064-006-9088-9
- ISSN
- 0364-3190
1573-6903
- Abstract
- 1-Methyl-4-phenylpyridinium (MPP+) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel blocker), EGTA (an extracellular calcium chelator), BAPTA-AM (a cell permeable calcium chelator) and calmodulin antagonists (W-7 and calmidazolium) attenuated the MPP+-induced mitochondrial damage and cell death. In contrast, the compounds did not reduce the toxicity of 6-OHDA. Treatment with MPP+ or 6-OHDA evoked the elevation of intracellular Ca2+ levels. Unlike cell injury, addition of nicardipine, BAPTA-AM and calmodulin antagonists prevented the elevation of intracellular Ca(2+)levels due to both toxins. The results show that the MPP+-induced formation of the mitochondrial permeability transition seems to be mediated by elevation of intracellular Ca2+ levels and calmodulin action. In contrast, the 6-OHDA-induced cell death seems to be mediated by Ca2+-independent manner.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24310)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.