Analysis of beauvericin and unusual enniatins co-produced by Fusarium oxysporum FB1501 (KFCC 11363P)
- Authors
- Song, Hyuk-Hwan; Ahn, Joong-Hoon; Lim, Yoong Ho; Lee, Chan
- Issue Date
- Jul-2006
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- cyclodepsipeptide; beauvericin; enniatin H; enniatin I; enniatin MK1688; Fusarium oxysporum
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.16, no.7, pp 1111 - 1119
- Pages
- 9
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 16
- Number
- 7
- Start Page
- 1111
- End Page
- 1119
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24316
- ISSN
- 1017-7825
1738-8872
- Abstract
- Beauvericins and enniatins are cyclohexadepsipeptides exhibiting various biological activities on animal systems, including humans. Fusarium oxysporum FB1501 (KFCC 11363P) that produces four different cyclohexadepsipeptides was isolated from soil in Korea and the structures of the four cyclohexadepsipeptides elucidated by HPLC, MS, IR, and NMR analyses. The molecular weights for compounds 1, 2, 3, and 4 were determined to be 654.5, 784.5, 668.6, and 682.5, respectively, on the basis of ESI-MS measurements. The IR spectra for all the compounds exhibited absorptions for ester (1,733-1,743cm(-1)) and amide (1,649-1,655cm(-1)) bonds that were very similar to those for beauvericin and enniatins with ester and amide absorptions. The results of the NMR analysis (H-1, C-13, 135-DEPT, COSY, HMQC, and HMBC; in CDCl3) revealed that compounds 1, 3), and 4 consisted Of L-N-methyl valine (N-MeVal), D-alpha-hydroxyisovaleic acid (Hiv), and 2-hydroxy-3-methylpentanoic acid (Hmp) residues (compound 1: three N-MeVal residues, two Hiv residues, and one Hmp residue; compound 3: three N-MeVal residues, one Hiv, and two Hmp residues; compound 4: three N-MeVal residues and three Hmp residues). Therefore, the compounds were identified as enniatin H (compound 1), enniatin I (compound 3), and enniatin MK1688 (compound 4). Compound 2 was analyzed as beauvericin according to ID and 2D NMR analyses. This study is the first report related to the co-production of beauvericin with other unusual enniatins, such as enniatin H, enniatin 1, and enniatin MK1688, by Fusarium oxysporum.
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