Electrically stimulated relaxation is not mediated by GABA in cat lower esophageal sphincter muscle
- Authors
- Park, SY; Shin, CY; Song, HJ; Min, YS; La, HO; Lee, JW; Kim, DY; Je, HD; Sohn, UD
- Issue Date
- May-2006
- Publisher
- PHARMACEUTICAL SOCIETY KOREA
- Keywords
- lower esophageal sphincter; GABA; electrical field stimulation; nonadrenergic noncholinergic neuron; nitric oxide; relaxation
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.29, no.5, pp 400 - 404
- Pages
- 5
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 29
- Number
- 5
- Start Page
- 400
- End Page
- 404
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24365
- DOI
- 10.1007/BF02968590
- ISSN
- 0253-6269
1976-3786
- Abstract
- This study examined the effect of Gamma-Amino butyric acid (GABA) and selective GABA receptor related drugs on the electrically stimulated relaxation in the lower esophageal sphincter muscle (LES) of a cat. Tetrodotoxin (10(-6) M) suppressed the electrically stimulated (0.5-5 Hz) relaxation of the LES. However, guanethidine (10(-6) M) and atropine (10(-6) M) had no effect indicating that the relaxations were neurally mediated via the nonadrenergic and noncholinergic (NANC) pathways. NG-nitro-L-arginine methyl ester (10(-4) M, L-NAME) also inhibited the relaxant response but did not completely abolish the electrically stimulated relaxation with 60 % inhibition, which suggests the involvement of nitric oxide as an inhibitory transmitter. This study examined the role of GABA, an inhibitory neurotransmitter, on neurally mediated LES relaxation. GABA (10(-3)-10(-5) M, non selective receptor agonist), muscimol (10(-3)-10(-5) M, GABA-A agonist), and baclofen (10(-3)-10(-3) M, GABA-B agonist) had no significant effect on the electrically stimulated relaxation. Moreover, bicuculline (10(-5) M, GABA-A antagonist) and phaclofen (10(-5) M, GABA-B antagonist) had no inhibitory effect on the electrically stimulated relaxation. This suggests that GABA and the GABA receptor are not involved in the electrically stimulated NANC relaxation in the cat LES.
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