HPLC determination of tolperisone in human plasma
- Authors
- Bae, JW; Park, YS; Sohn, UD; Myung, CS; Ryu, BK; Jang, CG; Lee, SY
- Issue Date
- Apr-2006
- Publisher
- PHARMACEUTICAL SOCIETY KOREA
- Keywords
- tolperisone; plasma; HPLC; pharmacokinetics
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.29, no.4, pp 339 - 342
- Pages
- 4
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 29
- Number
- 4
- Start Page
- 339
- End Page
- 342
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24385
- DOI
- 10.1007/BF02968581
- ISSN
- 0253-6269
1976-3786
- Abstract
- A simple high performance liquid chromatographic (HPLC) method was developed for the determination of tolperisone in human plasma. Tolperisone and internal standard (chlorphenesin) were isolated from 1 mL of plasma using 8 mL of dichlormethane. The organic phase was collected and evaporated under nitrogen gas. The residue was then reconstituted with 300 mL aliquot of mobile phase and a 100 mL aliquot was injected onto the Cla reverse-phased column. The mobile phase, 45% methanol containing 1% glacial acetic acid and 0.05% 1-hexanesulfonic acid was run at a flow rate of 1 mL/min. The column effluent was monitored using UV detector at 260 nm. The retention times for tolperisone and the internal standard were approximately 7.1 and 8.4 min, respectively. The standard curve was linear with minimal intraday and inter-day variability. The quantification limit of tolperisone in human plasma was 10 ng/ mL. The proposed method has been applied to the determination of pharmacolkinetic profile of tolperisone in Koreans. The T-max of tolperisone in Koreans (0.94 +/- 0.42 h) was not significantly differ from that reported in Europeans (0.5-1 h), but the mean half-life in Koreans (1.14 +/- 0.27 h) was shorter than that in Europeans (2.56 +/- 0.2 h). The proposed HPLC method is simple, accurate, reproducible and suitable for pharmacokinetic study of tolperisone.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24385)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.