IL-1 beta activates p44/42 and p38 mitogen-activated protein kinases via different pathways in cat esophageal smooth muscle cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Tai Sang | - |
dc.contributor.author | Song, Hyun Ju | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Min, Young Sil | - |
dc.contributor.author | Shin, Chang Yell | - |
dc.contributor.author | Sohn, Uy Dong | - |
dc.date.available | 2019-05-30T07:33:45Z | - |
dc.date.issued | 2006-02 | - |
dc.identifier.issn | 1007-9327 | - |
dc.identifier.issn | 2219-2840 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24411 | - |
dc.description.abstract | AIM: To examine the pathway related to the IL-1 beta-induced activation of mitogen-activated protein (MAP) kinases in cat esophageal smooth muscle cells. METHODS: Culture of the esophageal smooth muscle cells from cat was prepared. Specific inhibitors were treated before applying the IL-1 beta. Western blot analysis was performed to detect the expressions of COX, iNOS and MAP kinases. RESULTS: In the primary cultured cells, although IL-1 beta failed to upregulate the COX and iNOS levels, the levels of the phosphorylated forms of p44/42 MAP kinase and p38 MAP kinase increased in both concentration- and time-dependent manner, of which the level of activation reached a maximum within 3 and 18 h, respectively. The pertussis toxin reduced the level of p44/42 MAP kinase phosphorylation. Tyrphostin 51 and genistein also inhibited this activation. Neomycin decreased the density of the p44/42 MAP kinase band to the basal level. Phosphokinase C (PKC) was found to play a mediating role in the IL-1 beta-induced p44/42 MAP kinase activity. In contrast, the activation of p38 MAP kinase was inhibited only by a pretreatment with forskolin, and was unaffected by the other compounds. CONCLUSION: Based on these results, IL-1 beta-induced p44/42 MAP kinase activation is mediated by the Gi protein, tyrosine kinase, phospholipase C (PLC) and PKC. The pathway for p38 MAP kinase phosphorylation is different from that of p44/42 MAP kinase, suggesting that it plays a different role in the cellular response to IL-1 beta. (C) 2006 The WJG Press. All rights reserved. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | W J G PRESS | - |
dc.title | IL-1 beta activates p44/42 and p38 mitogen-activated protein kinases via different pathways in cat esophageal smooth muscle cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3748/wjg.v12.i5.716 | - |
dc.identifier.bibliographicCitation | WORLD JOURNAL OF GASTROENTEROLOGY, v.12, no.5, pp 716 - 722 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000239947900008 | - |
dc.identifier.scopusid | 2-s2.0-33644888001 | - |
dc.citation.endPage | 722 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 716 | - |
dc.citation.title | WORLD JOURNAL OF GASTROENTEROLOGY | - |
dc.citation.volume | 12 | - |
dc.type.docType | Article | - |
dc.publisher.location | 중국 | - |
dc.subject.keywordAuthor | IL-1 beta | - |
dc.subject.keywordAuthor | MAP kinase | - |
dc.subject.keywordAuthor | esophageal smooth muscle cells | - |
dc.subject.keywordPlus | NECROSIS-FACTOR-ALPHA | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | CYCLOOXYGENASE-2 EXPRESSION | - |
dc.subject.keywordPlus | GASTROESOPHAGEAL REFLUX | - |
dc.subject.keywordPlus | RECEPTOR ANTAGONIST | - |
dc.subject.keywordPlus | SIGNAL-TRANSDUCTION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | GASTRIC-MUCOSA | - |
dc.subject.keywordPlus | INTERLEUKIN-1 | - |
dc.subject.keywordPlus | DISEASE | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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