IL-1 beta activates p44/42 and p38 mitogen-activated protein kinases via different pathways in cat esophageal smooth muscle cells
- Authors
- Lee, Tai Sang; Song, Hyun Ju; Jeong, Ji Hoon; Min, Young Sil; Shin, Chang Yell; Sohn, Uy Dong
- Issue Date
- Feb-2006
- Publisher
- W J G PRESS
- Keywords
- IL-1 beta; MAP kinase; esophageal smooth muscle cells
- Citation
- WORLD JOURNAL OF GASTROENTEROLOGY, v.12, no.5, pp 716 - 722
- Pages
- 7
- Journal Title
- WORLD JOURNAL OF GASTROENTEROLOGY
- Volume
- 12
- Number
- 5
- Start Page
- 716
- End Page
- 722
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24411
- DOI
- 10.3748/wjg.v12.i5.716
- ISSN
- 1007-9327
2219-2840
- Abstract
- AIM: To examine the pathway related to the IL-1 beta-induced activation of mitogen-activated protein (MAP) kinases in cat esophageal smooth muscle cells. METHODS: Culture of the esophageal smooth muscle cells from cat was prepared. Specific inhibitors were treated before applying the IL-1 beta. Western blot analysis was performed to detect the expressions of COX, iNOS and MAP kinases. RESULTS: In the primary cultured cells, although IL-1 beta failed to upregulate the COX and iNOS levels, the levels of the phosphorylated forms of p44/42 MAP kinase and p38 MAP kinase increased in both concentration- and time-dependent manner, of which the level of activation reached a maximum within 3 and 18 h, respectively. The pertussis toxin reduced the level of p44/42 MAP kinase phosphorylation. Tyrphostin 51 and genistein also inhibited this activation. Neomycin decreased the density of the p44/42 MAP kinase band to the basal level. Phosphokinase C (PKC) was found to play a mediating role in the IL-1 beta-induced p44/42 MAP kinase activity. In contrast, the activation of p38 MAP kinase was inhibited only by a pretreatment with forskolin, and was unaffected by the other compounds. CONCLUSION: Based on these results, IL-1 beta-induced p44/42 MAP kinase activation is mediated by the Gi protein, tyrosine kinase, phospholipase C (PLC) and PKC. The pathway for p38 MAP kinase phosphorylation is different from that of p44/42 MAP kinase, suggesting that it plays a different role in the cellular response to IL-1 beta. (C) 2006 The WJG Press. All rights reserved.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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