Rapid and highly sensitive liquid chromatography/electrospray ionization tandem mass spectrometry method for the quantitation of buspirone in human plasma: application to a pharmacokinetic study
- Authors
- Cho, SH; Lee, HW; Im, HT; Park, WS; Choi, YW; Rew, JH; Lee, KT
- Issue Date
- 2006
- Publisher
- JOHN WILEY & SONS LTD
- Citation
- RAPID COMMUNICATIONS IN MASS SPECTROMETRY, v.20, no.8, pp 1293 - 1298
- Pages
- 6
- Journal Title
- RAPID COMMUNICATIONS IN MASS SPECTROMETRY
- Volume
- 20
- Number
- 8
- Start Page
- 1293
- End Page
- 1298
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24451
- DOI
- 10.1002/rcm.2441
- ISSN
- 0951-4198
1097-0231
- Abstract
- A rapid and sensitive method for the quantitation of buspirone in human plasma by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was developed. Plasma samples were treated by liquid-liquid extraction with methyl tert-butyl ether (MTBE). The chromatographic separation was performed isocratically on a reversed-phase Shiseido C-18 column (50 mm X 2.0 mm, 3 mu m) with a mobile phase of acetonitrile/0.1% acetic acid (1:1, v/v). The acquisition was performed in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 386 -> 122 for buspirone and m/z 409 -> 238 for amlodipine (the internal standard). The method was validated to determine its specificity, recovery, limit of quantitation, accuracy and precision. The lower limit of quantitation was 0.02 ng/mL with a relative standard deviation of less than 10%. The present method provides an accurate, precise and sensitive tool for buspirone and was successfully applied to a pharmacokinetic study in eight subjects. Copyright (c) 2006 John Wiley & Sons, Ltd.
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