Reversal of silver sulfadiazine-impaired wound healing by epidermal growth factor
- Authors
- Lee, ARC; Leem, H; Lee, J; Park, KC
- Issue Date
- Aug-2005
- Publisher
- ELSEVIER SCI LTD
- Keywords
- antibacterial; growth factors; keratinocyte; cytotoxicity; wound healing; collagen
- Citation
- BIOMATERIALS, v.26, no.22, pp 4670 - 4676
- Pages
- 7
- Journal Title
- BIOMATERIALS
- Volume
- 26
- Number
- 22
- Start Page
- 4670
- End Page
- 4676
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24558
- DOI
- 10.1016/j.biomaterials.2004.11.041
- ISSN
- 0142-9612
1878-5905
- Abstract
- Silver sulfadiazine (Ag-SD) is a useful antibacterial agent for wound treatment. However, recent findings indicate that the compound delays the wound-healing process. That delay may be reversed by treatment with growth factors. The purpose of this study, was to evaluate the cyto-protective effect of epidermal growth factor (EGF) against Ag-SD treated keratinocytes and to investigate the reversibility of the impaired wound-healing process by the co-supplementation of EGF. Four types of drug-loaded collagen sponge dressings with different concentrations of Ag-SD, EGF and Ag-SD+EGF were prepared. An immortalized keratinocyte, HaCaT cells, were cultured in 35-mm Petri-dish using Dulbecco's Modified Eagle's Minimal Essential Medium (DMEM) with 10% FBS. Cultures were treated with the samples Submerged, and viabilities of cultures were evaluated using MTT assay. The wound heal efficacy was evaluated in a partial thickness burn mouse model. Cells treated with EGF showed a cytoprotective effect on 1% Ag-SD treated cells with significant increase in viable cell numbers at concentrations ranging from I to 50 mu g/ml. The cytotoxicity of Ag-SD impaired wound healing, while the addition of EGF could reverse the impairment. This evidence suggests that EGF is a useful agent in the retardation of wound healing caused by Ag-SD. Therefore, a drug delivery system containing both EGF and Ag-SD, such as that used in the study, may be clinically relevant. (c) 2004 Published by Elsevier Ltd.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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