Enhancement of erythropoietin production from Chinese hamster ovary (CHO) cells by introduction of the urea cycle enzymes, carbamoyl phosphate synthetase I and ornithine transcarbamylase
- Authors
- Kim, N; Lee, Y; Kim, H; Choi, J; Kim, J; Chang, KH; Kim, JH; Kim, HJ
- Issue Date
- Aug-2004
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- erythropoietin; urea cycle enzymes; ammonia concentration; sialylation
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.14, no.4, pp 844 - 851
- Pages
- 8
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 14
- Number
- 4
- Start Page
- 844
- End Page
- 851
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/24801
- ISSN
- 1017-7825
1738-8872
- Abstract
- Efficient mammalian erythropoietin (EPO)-expression systems are required for therapeutic applications. The accumulation of ammonia is a major problem in the production of recombinant proteins in cultured animal cells. To counter this problem we introduced the first two genes of the urea cycle, carbamoyl phosphate synthetase (CPSI) and ornithine transcarbamylase (OTC), into IBE Chinese Hamster Ovary (CHO) cells by stable transfection. The resulting cell line, CO5, had a higher growth rate and accumulated less ammonia per cell than the parental cell line, IBE. In addition, it produced 2 times more EPO than the parent, and the purified EPO contained a higher proportion of acidic isoforms with approximately 15% more sialic acid.
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