Induction of apoptosis by 3,4 '-dimethoxy-5-hydroxystilbene in human promyeloid leukemic HL-60 cells
- Authors
- Lee, SH; Ryu, SY; Kim, HB; Kim, MY; Chun, YJ
- Issue Date
- Feb-2002
- Publisher
- GEORG THIEME VERLAG KG
- Keywords
- hydroxystilbene; apoptosis; HL-60 cells; caspases; Bax; cytochrome c
- Citation
- PLANTA MEDICA, v.68, no.2, pp 123 - 127
- Pages
- 5
- Journal Title
- PLANTA MEDICA
- Volume
- 68
- Number
- 2
- Start Page
- 123
- End Page
- 127
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25144
- DOI
- 10.1055/s-2002-20242
- ISSN
- 0032-0943
1439-0221
- Abstract
- 3,4'-Dimethoxy-5-hydroxystilbene (DMHS) is a hydroxystilbene compound obtained by methylation and acid hydrolysis of piceid (resveratrol-3-0-glucoside) from Polygonum cuspidatum. Herein, we report that DMHS induces programmed cell death or apoptosis in human promyelocytic leukemic HL-60 cells. We found that treatment of HL-60 cells with DMHS suppressed the cell growth in a concentration-dependent manner with an IC50 value of 25,muM. DMHS increased internucleosomal DNA fragmentation in a time-dependent manner. The cell death by DMHS was partially prevented by the caspase inhibitor, zVAD-fmk. DMHS caused activation of caspases such as caspase-3, -8, and -9. Immunoblot experiments revealed that DMHS-induced apoptosis was associated with the induction of Bax expression. The release of cytochrome c from mitochondria into the cytosol was increased in response to DMHS. Taken together, our present results indicated that DMHS leads to apoptotic cell death in HL-60 cells through increased Bax expression and release of cytochrome c into cytosol and may be considered as a good candidate for a cancer chemopreventive agent in humans.
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