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Operational characteristics of an antibody-immobilized QCM system detecting Salmonella spp.
- Issue Date
- Jun-2000
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- quartz crystal microbalance system; anti-Salmonella antibody immobilization; sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate; thiolation; Salmonella detection; model sample measurement
- Citation
- BIOSENSORS & BIOELECTRONICS, v.15, no.3-4, pp 167 - 172
- Pages
- 6
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 15
- Number
- 3-4
- Start Page
- 167
- End Page
- 172
- ISSN
- 0956-5663
1873-4235
- Abstract
- A quartz crystal microbalance (QCM) system detecting Salmonella spp. was developed by an anti-Salmonella antibody immobilization onto one gold surface of a piezoelectric quartz crystal surface with sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate (sulfo-LC-SPDP) thiolation. The optimum temperature and pH for the antibody-immobilized sensor were 35 degrees C and 7.2, respectively. The frequency shifts obtained were correlated with the Salmonella concentrations in the range 3.2 x 10(6)-4.8 x 10(8) CFU per ml. The system was quite specific to Salmonella spp. and applicable for repetitive use after a regeneration step employing 1.2 M NaOH. A model sample measurement was done for a market milk spiked with Salmonella typhimurium. (C) 2000 Elsevier Science S.A. All rights reserved.
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