Improvement of a recombinant anti-monkey anti-CD3 diphtheria toxin based immunotoxin by yeast display affinity maturation of the scFv
DC Field | Value | Language |
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dc.contributor.author | Wang, Zhirui | - |
dc.contributor.author | Kim, Geun-Bae | - |
dc.contributor.author | Woo, Jung-Hee | - |
dc.contributor.author | Liu, Yuan Yi | - |
dc.contributor.author | Mathias, Askale | - |
dc.contributor.author | Stavrou, Scott | - |
dc.contributor.author | Neville Jr., David M. | - |
dc.date.available | 2019-06-10T05:31:10Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1043-1802 | - |
dc.identifier.issn | 1520-4812 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25474 | - |
dc.description.abstract | Recently, a bivalent recombinant anti-human CD3 diphtheria toxin (DT) based immunotoxin derived from the scFv of UCHT1 antibody has been made that shows enhanced bioactivity and is free from the side effects of Fc receptor interaction. In this case, the diminution of CD3 binding due to the placement of the scFv domain at the C-terminus of the truncated DT in single scFv immunotoxins was compensated by adding an additional scFv domain. However, this strategy was less successful for constructing an anti-rhesus recombinant immunotoxin derived from the scFv of FN18 antibody due to poor binding of the anti-rhesus bivalent immunotoxin. We report here that, by increasing the FN18 scFv affinity through random mutagenesis and selection with a dye-labeled monkey CD3εγ recombinant heterodimer, we greatly improved the bioactivity of FN18 derived immunotoxin. The best mutant, C207, contained nine mutations, two of which were located in CDRs that changed the charge from negative to positive. Binding affinity of the C207 scFv to the monkey T cell line HSC-F increased 9.8-fold. The potency of the C207 bivalent immunotoxin assayed by inhibition of protein synthesis increased by 238-fold. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | American Chemical Society | - |
dc.title | Improvement of a recombinant anti-monkey anti-CD3 diphtheria toxin based immunotoxin by yeast display affinity maturation of the scFv | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/bc0603438 | - |
dc.identifier.bibliographicCitation | Bioconjugate Chemistry, v.18, no.3, pp 947 - 955 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-34347337714 | - |
dc.citation.endPage | 955 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 947 | - |
dc.citation.title | Bioconjugate Chemistry | - |
dc.citation.volume | 18 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | diphtheria toxin | - |
dc.subject.keywordPlus | Fc receptor | - |
dc.subject.keywordPlus | immunotoxin | - |
dc.subject.keywordPlus | recombinant human CD3 diphtheria toxin antibody | - |
dc.subject.keywordPlus | ucht1 antibody | - |
dc.subject.keywordPlus | unclassified drug | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | binding affinity | - |
dc.subject.keywordPlus | carboxy terminal sequence | - |
dc.subject.keywordPlus | diphtheria | - |
dc.subject.keywordPlus | immunotoxicity | - |
dc.subject.keywordPlus | mutagenesis | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | protein synthesis inhibition | - |
dc.subject.keywordPlus | T lymphocyte activation | - |
dc.subject.keywordPlus | toxin analysis | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Antibody Affinity | - |
dc.subject.keywordPlus | Antigens, CD3 | - |
dc.subject.keywordPlus | Cells, Cultured | - |
dc.subject.keywordPlus | Codon | - |
dc.subject.keywordPlus | Dimerization | - |
dc.subject.keywordPlus | Diphtheria Toxin | - |
dc.subject.keywordPlus | Haplorhini | - |
dc.subject.keywordPlus | Immunoglobulin Variable Region | - |
dc.subject.keywordPlus | Immunotoxins | - |
dc.subject.keywordPlus | Mutagenesis | - |
dc.subject.keywordPlus | Mutation | - |
dc.subject.keywordPlus | Pichia | - |
dc.subject.keywordPlus | Recombinant Proteins | - |
dc.subject.keywordPlus | Rh-Hr Blood-Group System | - |
dc.subject.keywordPlus | Saccharomyces cerevisiae | - |
dc.subject.keywordPlus | T-Lymphocytes | - |
dc.description.journalRegisteredClass | scopus | - |
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