Effects of granulocyte macrophage colony-stimulating factor (GM-CSF) on the interleukin-6 expression in the prostate cancer cell line PC-3
- Authors
- Kang, G.H.; Myung, S.C.; Kim, T.H.; Oh, S.Y.; Won, E.H.; Kim, S.C.; Kim, W.S.; Kim, Y.S.
- Issue Date
- Jul-2006
- Publisher
- Korean Urological Association
- Keywords
- Granulocyte-macrophage colony-stimulating factor; Interleukin-6; Prostate cancer
- Citation
- Korean Journal of Urology, v.47, no.7, pp 786 - 790
- Pages
- 5
- Journal Title
- Korean Journal of Urology
- Volume
- 47
- Number
- 7
- Start Page
- 786
- End Page
- 790
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25536
- DOI
- 10.4111/kju.2006.47.7.786
- ISSN
- 0494-4747
2466-054X
- Abstract
- Purpose: Interleukin-6 (IL-6) can stimulate a variety of tumors including prostatic carcinoma. Research has recently shown that IL-6 may act to stimulate the progression of prostatic cancer. IL-6 is elevated in the sera of patients with metastatic prostatic cancer and it has been shown to be a candidate marker of disease activity. To date, little work has been performed to characterize the nature of granulocyte macrophage colony-stimulating factor (GM-CSF) and the expression of IL-6. The aim of this study is to evaluate the effects of GM-CSF on the expression of IL-6 in PC-3 cells. Materials and Methods: The bone-derived PC-3 cell line was used in this study. Reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the GM-CSF and also the IL-6 mRNA expression. The IL-6 protein was measured by enzyme-linked immunosorbent assay (ELISA) after treatments with the hGM-CSF. Results: hGM-CSF was expressed in the PC-3 cell line. Our data indicated that the IL-6 mRNA expression was not increased at 4, 8 and 12 hours by the hGM-CSF in comparison to the control group, but it was slightly increased at 24 and 48 hours. The expression of IL-6 protein was increased at 4, 8, 12, 24 and 48 hours after hGM-CSF treatment, in comparison with the control group. Conclusions: The IL-6 mRNA expression was slightly increased by hGM-CSF at 24 and 48 hours in comparison to the control group. Yet the IL-6 protein expression increased before the IL-6 mRNA expression. Therefore, hGM-CSF may modulate the post-transcription pathway of the IL-6 expression in prostate carcinoma cells. Our data suggest that GM-CSF may have a possible IL-6 mediated pathophysiologic role in prostate cancer.
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