Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome
- Authors
- Gao, Junjun; Lee, Kangseok; Zhao, Meng; Qiu ,Ji; Zhan, Xiaoming; Saxena, Ankur; Moore, Christopher J.; Cohen, Stanley N.; Georgiou, George
- Issue Date
- Jul-2006
- Citation
- Molecular Microbiology, v.61, no.2, pp 394 - 406
- Pages
- 13
- Journal Title
- Molecular Microbiology
- Volume
- 61
- Number
- 2
- Start Page
- 394
- End Page
- 406
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25565
- DOI
- 10.1111/j.1365-2958.2006.05246.x
- ISSN
- 0950-382X
1365-2958
- Abstract
- In Escherichia coli the initial step in the processing or decay of many messenger and structural RNAs is mediated by the endonuclease RNase E, which forms the core of a large RNA-catalysis machine termed the degradosome. Previous experiments have identified a protein that globally modulates RNA abundance by binding to RNase E and regulating its endonucleolytic activity. Here we report the discovery of RraB, which interacts with a different site on RNase E and interferes with cleavage of a different set of transcripts. We show that expression of RraA or RraB in vivo is accompanied by dramatic, distinct, and inhibitor-specific changes in degradosome composition - and that these are in turn associated with alterations in RNA decay and global transcript abundance profiles that are dissimilar to the profile observed during simple RNase E deficiency. Our results reveal the existence of endonuclease binding proteins that modulate the remodelling of degradosome composition in bacteria and argue that such degradosome remodelling is a mechanism for the differential regulation of RNA cleavages in E. coli.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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