Overexpression of USF increases TGF-β1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells
DC Field | Value | Language |
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dc.contributor.author | Kim, K.-S. | - |
dc.contributor.author | Hye, S.J. | - |
dc.contributor.author | Yun, J.C. | - |
dc.contributor.author | Tae, S.J. | - |
dc.contributor.author | Hye, W.J. | - |
dc.contributor.author | Lee, M.-S. | - |
dc.contributor.author | Kim, K.-W. | - |
dc.contributor.author | Jae, H.C. | - |
dc.date.available | 2019-06-11T08:32:41Z | - |
dc.date.issued | 2008-10 | - |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.issn | 1598-6357 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25640 | - |
dc.description.abstract | Transforming growth factor-β1 (TGF-β1) is a potent inhibitor of cellular growth and proliferation by G1 phase arrest or apoptosis. We investigated the association of TGF-β1 with the anti-proliferative effect of upstream stimulatory factor (USF) in Fischer rat thyroid cell line (FRTL-5) cells. [Methyl-3H] thymidine uptake was measured after treatment of FRTL-5 cells with TGF-β1 to identify its anti-proliferative effect. USF-1 and USF-2 proteins were in vitro translated, and an electrophoretic mobility shift assay was performed to identify the interaction between USF and the TGF-β1 promoter. FRTL-5 cells were transfected with USF cDNA, and then the expression of TGF-β1 was examined with Northern and Western blotting. The cell cycle-regulating proteins associated with TGF-β1 were also measured. TGF-β1 significantly inhibited [methyl-3H] thymidine uptake in FRTL-5 cells. Two specific binding sites for USF were found in the TGF-β1 promoter: -1,846∼-1,841 (CACATG) and -621∼-616 (CATGTG). Overexpression of USF increased both the mRNA levels and protein levels of TGF-β1. However, the expression of cyclin D1, CDK4, cyclin E, and CDK2, and the phosphorylation of retinoblastoma protein remained unchanged. Overexpression of USF in FRTL-5 cells increased the expression of TGF-β10 through specific binding to TGF-β1 promoter. However, the USF-induced expression of TGF-β1 did not cause G1 arrest. Copyright ©The Korean Academy of Medical Sciences. | - |
dc.format.extent | 7 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN ACAD MEDICAL SCIENCES | - |
dc.title | Overexpression of USF increases TGF-β1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3346/jkms.2008.23.5.870 | - |
dc.identifier.bibliographicCitation | Journal of Korean Medical Science, v.23, no.5, pp 870 - 876 | - |
dc.identifier.kciid | ART001287248 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000260724900020 | - |
dc.identifier.scopusid | 2-s2.0-56049113294 | - |
dc.citation.endPage | 876 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 870 | - |
dc.citation.title | Journal of Korean Medical Science | - |
dc.citation.volume | 23 | - |
dc.type.docType | Article | - |
dc.publisher.location | 대한민국 | - |
dc.subject.keywordAuthor | FRTL-5 cells | - |
dc.subject.keywordAuthor | Transforming growth factor beta1 | - |
dc.subject.keywordAuthor | Upstream stimulatory factor | - |
dc.subject.keywordPlus | thymidine | - |
dc.subject.keywordPlus | transforming growth factor beta1 | - |
dc.subject.keywordPlus | upstream stimulatory factor | - |
dc.subject.keywordPlus | Usf1 protein, rat | - |
dc.subject.keywordPlus | Usf2 protein, rat | - |
dc.subject.keywordPlus | animal | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | binding site | - |
dc.subject.keywordPlus | cell cycle | - |
dc.subject.keywordPlus | cell cycle G1 phase | - |
dc.subject.keywordPlus | cell line | - |
dc.subject.keywordPlus | chemistry | - |
dc.subject.keywordPlus | gene expression regulation | - |
dc.subject.keywordPlus | genetic transfection | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | promoter region | - |
dc.subject.keywordPlus | protein synthesis | - |
dc.subject.keywordPlus | rat | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Apoptosis | - |
dc.subject.keywordPlus | Binding Sites | - |
dc.subject.keywordPlus | Cell Cycle | - |
dc.subject.keywordPlus | Cell Line | - |
dc.subject.keywordPlus | G1 Phase | - |
dc.subject.keywordPlus | Gene Expression Regulation | - |
dc.subject.keywordPlus | Promoter Regions, Genetic | - |
dc.subject.keywordPlus | Protein Biosynthesis | - |
dc.subject.keywordPlus | Rats | - |
dc.subject.keywordPlus | Thymidine | - |
dc.subject.keywordPlus | Transfection | - |
dc.subject.keywordPlus | Transforming Growth Factor beta1 | - |
dc.subject.keywordPlus | Upstream Stimulatory Factors | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.description.journalRegisteredClass | scopus | - |
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