Overexpression of USF increases TGF-β1 protein levels, but G1 phase arrest was not induced in FRTL-5 cells
- Authors
- Kim, K.-S.; Hye, S.J.; Yun, J.C.; Tae, S.J.; Hye, W.J.; Lee, M.-S.; Kim, K.-W.; Jae, H.C.
- Issue Date
- Oct-2008
- Publisher
- KOREAN ACAD MEDICAL SCIENCES
- Keywords
- FRTL-5 cells; Transforming growth factor beta1; Upstream stimulatory factor
- Citation
- Journal of Korean Medical Science, v.23, no.5, pp 870 - 876
- Pages
- 7
- Journal Title
- Journal of Korean Medical Science
- Volume
- 23
- Number
- 5
- Start Page
- 870
- End Page
- 876
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/25640
- DOI
- 10.3346/jkms.2008.23.5.870
- ISSN
- 1011-8934
1598-6357
- Abstract
- Transforming growth factor-β1 (TGF-β1) is a potent inhibitor of cellular growth and proliferation by G1 phase arrest or apoptosis. We investigated the association of TGF-β1 with the anti-proliferative effect of upstream stimulatory factor (USF) in Fischer rat thyroid cell line (FRTL-5) cells. [Methyl-3H] thymidine uptake was measured after treatment of FRTL-5 cells with TGF-β1 to identify its anti-proliferative effect. USF-1 and USF-2 proteins were in vitro translated, and an electrophoretic mobility shift assay was performed to identify the interaction between USF and the TGF-β1 promoter. FRTL-5 cells were transfected with USF cDNA, and then the expression of TGF-β1 was examined with Northern and Western blotting. The cell cycle-regulating proteins associated with TGF-β1 were also measured. TGF-β1 significantly inhibited [methyl-3H] thymidine uptake in FRTL-5 cells. Two specific binding sites for USF were found in the TGF-β1 promoter: -1,846∼-1,841 (CACATG) and -621∼-616 (CATGTG). Overexpression of USF increased both the mRNA levels and protein levels of TGF-β1. However, the expression of cyclin D1, CDK4, cyclin E, and CDK2, and the phosphorylation of retinoblastoma protein remained unchanged. Overexpression of USF in FRTL-5 cells increased the expression of TGF-β10 through specific binding to TGF-β1 promoter. However, the USF-induced expression of TGF-β1 did not cause G1 arrest. Copyright ©The Korean Academy of Medical Sciences.
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