보통메밀과 쓴메밀의 산화적 스트레스 개선 및 위암 억제 효과

Abstract

The protective effects of tartary buckwheat and common Korean buckwheat cultivars, yangjul-maemil and daesan-maemil, against oxidative stress and gastric cancer were evaluated. The treatment of the methanol extract from tartary buckwheat, yangjul-maemil and daesan-maemil showed over 80% scavenging effect against 1,1-diphenyl-2-picrylhydrazyl at 250 μg/ml. In addition, all buckwheats showed over 80% ㆍOH scavenging activity even at low concentration of 50 μg/ml. In order to investigate the anti-inflammatory activity of tartary buckwheat, yangjul-maemil and daesan-maemil, we employed nitric oxide (NO) induced cellular inflammatory system using RAW 264.7 macrophage cells. The results showed that tartary buckwheat, yangjul-maemil and daesan-maemil inhibited NO production generated by both exotoxin of sodium nitroprusside and endotoxin of lipopolysaccharide & interferon-gamma. Morever,tartary buckwheat and daesan-maemil showed the strong antimicrobial activity (clear zone ＞14 mm)against Helicobacter pylori. Yangjul-maemil and daesan-maemil also inhibited the growth of AGS human gastric adenocarcinoma cells by up to 70% at concentration of 1,000 μg/ml. The present results indicate that buckwheats showed protective effects against oxidative stress and gastric cancer. (Cancer Prev Res 16, 249-254, 2011)