Quantitative Studies of Carbohydrate-protein Interaction using Functionalized Bacterial Spores in Solution and on Chips
- Authors
- Park, Tae Jung; Park, Jong Pil; Lee, Seok Jae; Lee, Sang Yup
- Issue Date
- Feb-2011
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- bacterial spore display; carbohydrate-protein interaction; concanavalin A; immobilization; lectin
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.16, no.1, pp 190 - 195
- Pages
- 6
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 16
- Number
- 1
- Start Page
- 190
- End Page
- 195
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/27832
- DOI
- 10.1007/s12257-010-0063-6
- ISSN
- 1226-8372
- Abstract
- Carbohydrate-protein interaction is one of the most important molecular events deemed critical for numerous biological processes. Therefore, understanding this interaction is essential. In this study, we used bacterial spore display techniques to present multiple copies of streptavidin on the surface of spores to explore carbohydrate-protein interaction in solution and on chips. By applying bacterial spores displaying streptavidin, we developed a new method which allows sensitive, versatile, and passive detection of carbohydrate-protein interactions with a 10-fold increase in sensitivity. The linear relationship of interactions between carbohydrates and labeled concanavalin A (con A) in solution and on functionalized bacterial spore chips has also been confirmed. To the best of our knowledge, this is the first example of development and characterization of binding behavior in carbohydrate-protein interactions using bacterial spore-displayed streptavidin. We believe this strategy may enable new high-throughput screening of carbohydrate interactions as well as establish a basis for monitoring inhibitors of carbohydrate-binding proteins when developing new drugs.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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