LC-ESI-MS/MS를 이용한 생체시료 중 브롬화피나베리움의 고감도 분석 및 이를 이용한 생체이용률 평가Sensitive Determination of Pinaverium Bromide in Human Plasma by
LC-ESI-MS/MS : Applicability to Oral Bioavailability Determination
- Authors
- 한상범; 박석; 이예리; 김호현; 이희주; 김윤균; 염정록
- Issue Date
- 2004
- Publisher
- 한국약제학회
- Keywords
- Pinaverium bromide; LC-ESI-MS/MS; Bioavailability; Pharmacokinetic parameters
- Citation
- Journal of Pharmaceutical Investigation, v.34, no.6, pp 513 - 519
- Pages
- 7
- Journal Title
- Journal of Pharmaceutical Investigation
- Volume
- 34
- Number
- 6
- Start Page
- 513
- End Page
- 519
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/28380
- ISSN
- 2093-5552
2093-6214
- Abstract
- A sensitive method for quantification of pinaverium bromide in human plasma was established using liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS/MS). Glimepiride was used as internal standard. Pinaverium bromide and internal standard in plasma sample were extracted using tert-butylmethylether(TBME). A centrifuged upper layer was then evaporated and reconstituted with mobile phase of acetonitrile-5 mM ammonium formate (80/20, pH 3.0). The reconstituted samples were injected into a C18 reversed-phase column. Using MS/MS with multiple reaction monitoring (MRM) mode, pinaverium and glimepirde were detected without severe interference from human plasma matrix. Pinaverium produced a protonated precursor ion ([M+H]+) at m/z 510.3 and a corresponding product ion at m/z 228.9. Internal standard produced a protonated precursor ion ([M+H]+) at m/z 491.5 and a corresponding product ion at m/z 352.0. Detection of pinaverium bromide in human plasma was accurate and precise, with limit of quantitation at 0.5 ng/ml. The method has been successfully applied to bioavailability study of pinaverium bromide tablet in Korean healthy male volunteers. Pharmacokinetic parameters such as AUCt, Cmax, Tmax, Kel and t1/2 were calculated.
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