Collagenase로 유도한 가토의 슬관절염에서 TGF-b1과 키토산-TGF-b1 복합체의 영향

Abstract

To evaluate the effects of the chitosan-TGF-β‚1 conjugate and TGF-β‚1 on osteoarthritic rabbit cartilage induced by collagenase type II. Chemical arthritis was induced in right knee of 15 rabbits by injection of collagenase type II(4 mg/ml, 0.25cc) twice at day 1and 4, and then each 5 rabbits treated by intra-articular injection of TGF-β‚1(15 ng/ml, 1 ml, group I), chitosan-TGF-β‚1 conjugate(Chitosan: 5.8 g/ml, TGF-β‚1: 15 ng/ml, group II), PBS(1ml, group III) at 10 days later in same knees. The appearance of the injected knee joints and lameness were observed weekly. The articular cartilage and synovium were obtained at 4 weeks were analysed by gross findings(India ink), histological examinations(H&E, Safrainin O) and evaluation through the Kikuchi score. The osteoarthritic sign about knee joints were the most severe at 1 weeks after the initiation of collagenase injections, but there were no difference of 3 groups in osteoarthritic sign and lameness is most severe in group 3. Group 2 showed the most superior results in gross findings using india ink, the above results is more prominent in evaluation of histological scoring(average scores of each group I : 14 ± 2.7, II : 10.3 ± 1.2, III : 20.3 ± 3.7). These results might suggest that, in the rabbit osteoarthritic knee model induced by collagenase, Chitosan-TGF-β‚1 conjugate is more effective than only TGF-β‚1 in inhibiting cartilage degeneration