형광과 여기광을 공간적으로 분리하는 바이오칩용 소형 형광측정시스템Miniature Biochip Fluorescence Detection System with Spatial Separation of Fluorescence from Excitation Light
- Authors
- 김호성; 김용권; 박주한; 이국녕; 최재호
- Issue Date
- 2005
- Publisher
- 대한전기학회
- Keywords
- 프리즘; 거울; 균일화; 공간적 분리; 소형 형광 측정 시스템
- Citation
- 전기학회논문지 C권, v.54, no.8(C), pp 378 - 383
- Pages
- 6
- Journal Title
- 전기학회논문지 C권
- Volume
- 54
- Number
- 8(C)
- Start Page
- 378
- End Page
- 383
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/29555
- ISSN
- 1229-246X
- Abstract
- We report the development of miniature fluorescence detection systems that employ miniature prism, mirrors and low coat CCD camera to detect the fluorescence emitted from 40 fluorescently-labeled protein patterns without scanner. This kind of miniature fluorescence detection systems can be used in point of care. We introduce two systems, one uses prism+mirror block and the other uses prism and two mirrors. A large NA microscope eyepiece and low cost CCD camera are used. We fabricated protein chip containing multi-pattern BSA labeled with Cy5, using MEMS technology and modified the surface chemically to clean and to immobilize proteins. The measurements show that the combination of prism and mirrors can homogenize elliptical excitation light over the sample with higher optical efficiency, and increase the separation between excitation and fluorescence light at the CCD to give higher signal intensity and higher signal to noise ratio. The measurements also show that protein concentrations ranging from 10 ng/ml to 1000 ng/ml can be assayed with very small error. We believe that the proposed fluorescence detection system can be refined to build a commercially valuable hand-held or miniature detection device.
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Collections - College of ICT Engineering > School of Electrical and Electronics Engineering > 1. Journal Articles
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