솔비나무 유래 렉틴의 정제 효율

Abstract

Mackia fauriei usingalkaline buffer on a fetuin-affinity column. Application of a borate-based elution buffer in the present study increased the spe-cific activity of purified lectin from crude protein extract by 2.6-fold, whilst only slightly decreasing the recovery by 1.13%.The biological properties of the lectin eluted with borate bufer were the same as those of the lectin eluted with alkalinebuffer, such as in terms of the hemaglutination activity, hemagglutination inhibition activity, molecular mass, purity, andcytotoxicity to human breast cancer cells. A prepared biotin-labeled lectin conjugate was used to investigate the binding tovarious glycoproteins. Our results indicate that eluting with borate buffer is more efficient than using alkaline buffer to iso-late the lectin adsorbed in a fetuin-affinity column.Keywords . lectin, Mackia fauriei, specific activity, glycoprotein.. (lectin). .... .. .... .. .... .... ... , .... ..... ... ... .. , ......... .. , .... ..... ..... .. . ... ... .. .. .1-3) ... .... .. .... D-galactose, D-mannose, N-acetylglucosamine, N-acetylgalactos-amine, L-fucose. .. ... . N-glycolylneuraminic acid. .. ... (silaic acid) ... , ..