저분자 키토산-TGF-β1 복합체가 사람 연골 세포의 시험관 내배양에 미치는 효과The Effect of LMC- TGF-β1 Conjugate on Proliferation and Production of Extracellular Matrix of Human Articular Chondrocytes in vitro
- Authors
- 장의찬; 송광섭; 강수용; 정호중; 양재준; 김경환; 이태진; 손태일
- Issue Date
- 2007
- Publisher
- 한국키틴키토산학회
- Keywords
- Human chondrocyte; Low Molecular weight Chitosan; TGF-β1; LMC-TGF-β1 conjugate
- Citation
- Journal of Chitin and Chitosan, v.12, no.4, pp 205 - 212
- Pages
- 8
- Journal Title
- Journal of Chitin and Chitosan
- Volume
- 12
- Number
- 4
- Start Page
- 205
- End Page
- 212
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/30846
- ISSN
- 1229-4160
- Abstract
- We aim to investigate the effects of LMC(Low Molecular weight Chitosan)- TGF-β1 conjugate on adult human chondrocytesin vitro. Primary chondrocytes were harvested from two patients who underwent total knee arthroplasty. The chondrocytes fromone patient were cultured in Mebiol gel??, 3 D culture system, for evaluation of cell proliferation and collagen type II expressionusing semi-quantitative RT-PCR. A monolayer culture for histological evaluation was performed with the Chondrocytesharvested from the other patient using H&E, safranin-O and immunohistochemistry stain(S-100 and type II collagen). Each culturewas divided into 4 experimental groups; group 1: control (PBS), group 2: chitosan(5.8g/mL), group 3: TGF-β1(15 ng/mL), group4: LMC-TGF-β1 conjugation(LMC 5.8g/mL, TGF-β1 15 ng/mL). In each group, conditioning was made only one time andsustained for 3days. Cell proliferation was sustained for 3 days and cell count declined slightly from day 6 in all groups.However, increased cell count was preserved well in the group 4, especially at day 3,6,9(p < 0.05) and in the group 3 and 4 attochemisty and RT-PCR revealed that Collagen type II synthesis was more enhanced with longerpreservation in the group 4 compared to other groups. The conjugation of TGF-β1 and LMC could have a more positive effecton the proliferation and production of type II collagen of human chondrocyte in vitro than using of single TGF-β1.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
- College of Medicine > College of Medicine > 1. Journal Articles
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