원조포미바이러스 U5 LTR 말단의 보존적인 잔기의 돌연변이에 대한 인테그라제의 반응성Reactivity of Prototype Foamy Virus Integrase to the Mutants of the Highly Conserved Terminal Sequence of U5 LTR
- Authors
- 현우석; 이동현; 고현탁; 신차균
- Issue Date
- 2008
- Publisher
- 대한약학회
- Keywords
- integrase; foamy virus; U5 LTR; conserved
- Citation
- 약 학 회 지, v.52, no.2, pp 125 - 130
- Pages
- 6
- Journal Title
- 약 학 회 지
- Volume
- 52
- Number
- 2
- Start Page
- 125
- End Page
- 130
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/30953
- ISSN
- 0377-9556
- Abstract
- The long terminal repeat (LTR) of retroviral DNA genome plays an important role in the integration process
by providing substrate recognition site for viral integrase (IN). The dinucleotide CA near the 3'-end of the LTR termini is
completely conserved among retoviruses. In order to study specificity of interaction between prototype foamy virus (PFV)
IN and its U5 LTR DNA, the effect of mutagenesis of the CA sequence was investigated by studying reactivity of PFV IN
to the mutant LTR substrates. Replacement of only the C or the A allowed 60 to 100% of the reactivity of the wild type
LTR substrate. In addition, replacement of the C and the A showed 50 to 80% of the reactivity of the wild type LTR substrate,
indicating that PFV IN has less specificity on the conserved CA sequence when it is compared to the other retroviral
INs. Therefore it is suggested that PFV IN is less dependent on the conserved sequence of LTR termini for its enzymatic
reaction.
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