Content analysis of rutin in the leaves of Boehmeria nivea harvested in different regions of South Korea by HPLC-UV
- Authors
- Cho, S.; Quilantang, N.G.; Lee, J.S.; Kim, Y.-M.; Kim, H.B.; Cho, E.J.; Jung, Y.-S.; Lee, S.
- Issue Date
- 2018
- Publisher
- Korean Society of Pharmacognosy
- Keywords
- Boehmeria nivea; Content analysis; HPLC-UV; Rutin
- Citation
- Natural Product Sciences, v.24, no.1, pp 36 - 39
- Pages
- 4
- Journal Title
- Natural Product Sciences
- Volume
- 24
- Number
- 1
- Start Page
- 36
- End Page
- 39
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/3174
- DOI
- 10.20307/nps.2018.24.1.36
- ISSN
- 1226-3907
- Abstract
- Phytochemical analysis of Boehmeria nivea (Bn) leaves by medium pressure liquid chromatography led to the isolation of a flavonoid glycoside identified by spectroscopic analysis as rutin. The amount of rutin in the leaves of Bn harvested from nine regions in South Korea (Bn 1-9) which were collected on the months of June, July, August, and September was determined by HPLC-UV analysis. A gradient elution program that utilizes a Discovery® C18 (4.6 × 250 mm, 5 µm) column and mobile phase composed of 1% acetic acid-water: acetonitrile (90:10 to 60:40 for min) was followed. The injection volume and flow rate were 10 µl and 1 mL/ min, respectively. UV detection was set at 350 nm. Results show that Bn-8 harvested in September reported the highest content of rutin among the samples analyzed. This study provides a basis for the optimal harvest time of Bn which maximizes the yield of rutin. © 2018, Korean Society of Pharmacognosy. All rights reserved.
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