ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans
DC Field | Value | Language |
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dc.contributor.author | Kieffer, Nicolas | - |
dc.contributor.author | Guzmán-Puche, Julia | - |
dc.contributor.author | Poirel, Laurent | - |
dc.contributor.author | Kang, Hyo Jung | - |
dc.contributor.author | Jeon, Che Ok | - |
dc.contributor.author | Nordmann, Patrice | - |
dc.date.available | 2019-08-13T06:00:04Z | - |
dc.date.issued | 2019-06 | - |
dc.identifier.issn | 1460-2091 | - |
dc.identifier.issn | 1460-2091 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/33156 | - |
dc.description.abstract | OBJECTIVES: Our aim was to characterize the putative MBL of the environmental strain Zhongshania aliphaticivorans isolated from a marine environment. METHODS: The putative MBL was identified in silico using the NCBI database. The β-lactamase gene was cloned into different Escherichia coli backgrounds. Kinetic parameters were determined using the purified enzyme. RESULTS: The enzyme named ZHO-1 shared 51% amino acid identity with the acquired class B carbapenemases IMP-1, KHM-1 and DIM-1. Expression of the blaZHO-1 gene in a susceptible E. coli resulted in a carbapenemase phenotype. Kinetic parameters determined from purified ZHO-1 enzyme showed that it had significant hydrolytic activity against most β-lactams including penicillins, cephalosporins and carbapenems, with the exception of aztreonam and cefepime. CONCLUSIONS: This study adds to the knowledge regarding environmental species as a reservoir of possible clinically relevant MBLs. © The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NLM (Medline) | - |
dc.title | ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans | - |
dc.type | Article | - |
dc.identifier.doi | 10.1093/jac/dkz057 | - |
dc.identifier.bibliographicCitation | The Journal of antimicrobial chemotherapy, v.74, no.6, pp 1568 - 1571 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000482076800017 | - |
dc.identifier.scopusid | 2-s2.0-85066867724 | - |
dc.citation.endPage | 1571 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1568 | - |
dc.citation.title | The Journal of antimicrobial chemotherapy | - |
dc.citation.volume | 74 | - |
dc.type.docType | Article | - |
dc.publisher.location | 영국 | - |
dc.relation.journalResearchArea | Infectious Diseases | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Infectious Diseases | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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